TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| JM109 | E. coli strain for transformation | TaKaRa |
| DH5α | E. coli strain for transformation | TaKaRa |
| S17-1 | Mobilizer strain | 14 |
| P. aeruginosa | ||
| PAO1 | Wild type | 36 |
| ΔccoN1 mutant | PAO1 with a markerless deletion of ccoN1 gene | This study |
| ΔccoN2 mutant | PAO1 with a markerless deletion of ccoN2 gene | This study |
| ΔccoN1 ΔccoN2 mutant | PAO1 with markerless deletions of ccoN1 and ccoN2 genes | This study |
| Δnir mutant | PAO1 with markerless deletions of nirS to nirN genes | 15 |
| ΔccoN1 ΔccoN2 Δnir mutant | PAO1 with markerless deletions of ccoN1, ccoN2, and nirS to nirN genes | This study |
| PS1700 | narG; 3,835 bp deleted from PA3874 to PA3876 | 12 |
| Plasmids | ||
| pHSG398 | Cloning vector; Cpr | TaKaRa |
| pG19II | pK19mobsac derived suicide vector; sacB Gmr | 13 |
| pG19ccoN1 | ccoN1 deletion cassette in pG19II | This study |
| pG19ccoN2 | ccoN2 deletion cassette in pG19II | This study |
| pG19nir | nirS-to-nirN deletion cassette in pG19II | 15 |
| pMEX9 | pME4510 derived promoter-probe vector; xylE Gmr | 8 |
| pMEXccoN1 | ccoN1 promoter region in pMEX9 | This study |
| pMEXccoN2 | ccoN2 promoter region in pMEX9 | This study |
a
Cpr, chloramphenicol resistant; Gmr, gentamicin resistant.