FIG 6.

Transcription of hpf, a ribosome dimer-promoting factor. (A) Graph depicting the relative transcript levels of hpf, including what appears to be a long 5′ untranslated region (5′UTR), as determined by analysis of RNA isolated from all growth stages, combined and subjected to RNA-seq. (i) and (ii) indicate the location of products analyzed by RT-PCR. (B) Semiquantitative RT-PCR analysis of the 5′UTR of hpf (i) and the hpf coding sequence (ii) in wild-type and RNase mutant strains using RNA isolated from early-exponential-phase cultures (OD₆₀₀ of ∼0.4). hrdB was used as a positive control for RNA abundance and RNA integrity, while no-RT reactions (using RNA as template) and no-template reactions served as negative controls, ensuring there was no DNA contamination of either RNA preparations or any PCR reagents.