TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype, description, or use | Reference(s) or source |
|---|---|---|
| Streptomyces venezuelae | ||
| ATCC 10712 | ||
| E325 | ATCC 10712 ΔSVEN_5265(rnc)::[aac(3)IV-oriT] | This work |
| E326 | ATCC 10712 ΔSVEN_5394(rnj)::[aac(3)IV-oriT] | This work |
| E. coli | ||
| DH5α | Plasmid construction and general subcloning | Invitrogen |
| ET12567/pUZ8002 | Generation of methylation-free plasmid DNA and conjugation into Streptomyces | 95, 96 |
| BW25113/pIJ790 | Construction of cosmid-based knockouts | 27 |
| Plasmids or cosmids | ||
| pIJ790 | Temperature-sensitive plasmid carrying λ-RED genes | 27 |
| pIJ82 | Integrating plasmid vector; complementation of mutant strains | Gift from H. Kieser |
| pIJ773 | Plasmid carrying the apramycin knockout cassette | 27 |
| 3B03 | S. venezuelae cosmid carrying SVEN_5265 (rnc) | Gift from M. Bibb and M. Buttner |
| 3J08 | S. venezuelae cosmid carrying SVEN_5394 (rnj) | Gift from M. Bibb and M. Buttner |
| pMC110 | pIJ82 + rnc; rnc complementation plasmid | This work |
| pMC111 | pIJ82 + rnj; rnj complementation plasmid | This work |