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Overexpression of rprA does not decrease CpxA or CpxR protein levels. The effect of overexpressing MicF and RprA on CpxA and CpxR protein levels was measured by Western blotting with primary antibodies against either CpxA (A) or CpxR (B), with a nonspecific band (NSB) as a loading control. Quantification of CpxA and CpxR bands was performed using a ChemiDoc MP imager (Bio-Rad) with Image Lab software and is shown beneath the images.
