FIG 1.

Secretion of the biofilm matrix proteins RbmC, Bap1, and RbmA is dependent on the T2S system. Concentrated culture supernatants of stationary-phase cultures and cell extracts from WT V. cholerae N16961 with vector only (WT/p), an epsD mutant with vector only (ΔepsD/p), and a complemented epsD mutant strain (ΔepsD/pEpsD) were loaded by equivalent optical densities. As controls, a strain with mutations in rbmA, rbmC, and bap1 (RΔABC) and its isogenic rugose parental strain (RA1552) were included. Primary detection was performed with anti-RbmC (1:1,000), anti-Bap1 (1:200), and anti-RbmA (1:1,000) antisera.