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Colony corrugation and pellicle formation are dependent on the T2S system. Shown are colony (top) and pellicle (bottom) phenotypes of rugose strain RA1552 with vector only (RA1552/p), RΔepsF with vector only (RΔepsF/p) or with the complementing plasmid (RΔepsF/pEpsF), and RΔrbmA Δbap1 ΔrbmC with vector only (RΔABC/p). Colonies were incubated for 4 days (bars, 1 mm), while pellicles were incubated for 3 days.
