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The T2S system does not secrete the DNase Dns. DNA zymography of culture supernatants from the WT V. cholerae strain N16961 and V. cholerae ΔepsD was performed. DNase activity is detected by the clearing of DNA from the SDS-PAGE gel. As controls, a strain with a mutation in dns and its isogenic parental strain (SA1552) were included. Molecular weight markers (in thousands) are indicated.
