FIG 5.

VPS is produced and secreted independently of the T2S system. VPS immunodot blot analysis was conducted with the WT strain N16961, the ΔepsD and Δeps mutants, the WT rugose strain RA1552, RΔepsF, RΔrbmA Δbap1 ΔrbmC (RΔABC), and a strain lacking VPS (RΔVPS). Dot blotting was carried out by spotting the soluble VPS fraction and cells onto nitrocellulose membranes. Undiluted samples (U) and dilutions are indicated. (Left) Incubation with anti-VPS (1:1,000) and HRP-conjugated goat anti-rabbit IgG (1:7,500). (Right) Secondary-antibody control.