FIG 6.

Growth on LB agar does not interfere with the function of the T2S system, and no leakage of a cytoplasmic marker is observed. Shown are data for soluble VPS fractions and cells from the WT V. cholerae strain N16961, the ΔepsD and Δeps mutants, the WT rugose strain RA1552, and the RΔepsF, RΔABC, and RΔVPS strains, with plasmids encoding either HA/P (pHA/P) or GFP (pGFP), grown on LB agar plates. (A) All samples were normalized to equivalent optical densities. Primary detection was performed with anti-HA/P (1:5,000) antiserum. Secondary detection was performed with HRP-conjugated goat anti-rabbit IgG (1:15,000). (B) GFP fluorescence was measured using excitation and emission wavelengths of 485 nm and 530 nm, respectively. Filled and open bars indicate fluorescence detected in cells and soluble VPS fractions, respectively. Results from three independent experiments are presented as average fluorescence units (FU)/OD₆₀₀ unit ± standard errors.