TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Properties | Source or reference |
|---|---|---|
| Vibrio cholerae strains | ||
| N16961 | WT El Tor O1 biotype | Laboratory collection |
| NΔepsD | N16961 ΔepsD; Kanr | 54 |
| NΔeps | N16961 ΔepsCDEFGHIJKLMN; Cmr | 54 |
| RA1552 | El Tor O1 biotype, rugose variant; spontaneous Rifr mutant of clinical isolate 92A1552 | 38 |
| RΔABC | Rugose variant; ΔrbmA Δbap1 ΔrbmC | 20 |
| RΔepsF | Rugose variant; ΔepsF | This study |
| RΔVPS | Δvps-I Δvps-II; rugose variant with deletion of vpsA to vpsK and vpsL to vpsQ; Rifr | 30 |
| SA1552 | El Tor O1 biotype, nonrugose | 38 |
| SΔdns | SA1552 Δdns | This study |
| TRH7000 | El Tor O1 biotype; thy Hgr ΔctxAB | Laboratory collection |
| TRHΔepsD | TRH7000 ΔepsC; Kanr | 28 |
| TRHΔepsG | TRH7000 ΔepsG; Kanr | 28 |
| Plasmids | ||
| pEpsD | epsD cloned into pMMB67; Ampr | 28 |
| pEpsG | epsG cloned into pMMB67; Ampr | 28 |
| pHA/P | ha/p cloned into pMMB66; Ampr | 55 |
| pGFP | gfpmut2 cloned into pMMB66; Ampr | 55 |
| pEpsF | epsF cloned into pMMB67; Ampr | This study |