FIG 1.

Growth profiles of the quadruple terminal oxidase mutant strains of P. aeruginosa. (A) Growth under aerobic conditions. The strains were cultivated in 40 ml LB medium in 300-ml Erlenmeyer flasks with shaking at 180 rpm. (B) Growth under microaerobic conditions (2% O₂). The strains were cultivated in 150 ml LB medium in 300-ml square bottles. The medium was bubbled with a gas mixture consisting of 2% O₂ and 98% N₂ at a gas flux rate of 150 ml/min. The data shown are representatives from at least three independent experiments. The same plots in linear scale are shown in Fig. S4 in the supplemental material.