TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| P. aeruginosa | ||
| PAO1ut | Our laboratory strain of PAO1 used as a wild type | 21 |
| QXBo | Deletion of cioAB, coxBA-coxC, ccoN1O1Q1P1, and ccoN2O2Q2P2 | This study |
| QXCi | Deletion of cyoABCDE, coxBA-coxC, ccoN1O1Q1P1, and ccoN2O2Q2P2 | This study |
| QXAa | Deletion of cyoABCDE, cioAB, ccoN1O1Q1P1, and ccoN2O2Q2P2 | This study |
| QXAaS2 | Suppressor mutant of QXAaS2; able to grow aerobically | This study |
| QXCb1 | Deletion of cyoABCDE, cioAB, coxBA-coxC, and ccoN2O2Q2P2 | This study |
| QXCb2 | Deletion of cyoABCDE, cioAB, coxBA-coxC, and ccoN1O1Q1P1 | This study |
| PTO5 | Deletion of cyoABCDE, cioAB, coxBA-coxC, ccoN1O1Q1P1, and ccoN2O2Q2P2 | This study |
| QXCb1Δcc4 | cc4 mutant of QXCb1 | This study |
| QXCb1ΔcycB | cycB mutant of QXCb1 | This study |
| QXCb1ΔPA4571 | PA4571 mutant of QXCb1 | This study |
| QXCb1ΔPA5491 | PA5491 mutant of QXCb1 | This study |
| QXCb2Δcc4 | cc4 mutant of QXCb2 | This study |
| QXCb2ΔcycB | cycB mutant of QXCb2 | This study |
| QXCb2ΔPA4571 | PA4571 mutant of QXCb2 | This study |
| QXCb2ΔPA5491 | PA5491 mutant of QXCb2 | This study |
| E. coli | ||
| JM109 | Host strain for DNA manipulation | 33 |
| S17-1 | C600::RP-4 2-(Tc::Mu)(Kan::Tn7) thi pro hsdR hsdM+ recA | 55 |
| MB43 | cydB cyoB appB nuoB, nonmarker derivative of MB44 | 43 |
| Plasmids | ||
| pUC18 | Cloning vector; Apr | 33 |
| pEX18Ap | Gene replacement vector; Apr Cbr oriT+ sacB+ | 34 |
| pFLP2 | Flp recombinase-expressing plasmid; Apr | 34 |
| pMMB67EH | IncQ, expression vector; Apr Cbr | 56 |
| pPS854tet | tet-FRT cassette, Apr Tcr | 35 |
| pEX-Δcyo | Plasmid for cyoABCDE mutation, a derivative of pEX18Ap, Tcr | This study |
| pEX-Δcio | Plasmid for cioAB mutation, a derivative of pEX18Ap | This study |
| pEX-Δcox | Plasmid for coxBA-coxC mutation, a derivative of pEX18Ap | This study |
| pEX-Δcco1 | Plasmid for ccoN1O1Q1P1 mutation, a derivative of pEX18Ap, Tcr | This study |
| pEX-Δcco2 | Plasmid for ccoN2O2Q2P2 mutation, a derivative of pEX18Ap, Tcr | This study |
| pEX-Δcc4 | Plasmid for cc4 mutation, a derivative of pEX18Ap | This study |
| pEX-ΔcycB | Plasmid for cycB mutation, a derivative of pEX18Ap | This study |
| pEX-ΔPA4571 | Plasmid for PA4571 mutation, a derivative of pEX18Ap | This study |
| pEX-ΔPA5491 | Plasmid for PA5491 mutation, a derivative of pEX18Ap | This study |
| pMMB-cc4 | cc4 on pMMB67EH | This study |
| pMMB-PA5491 | PA5491 on pMMB67EH | This study |
| pMMB-cioAB | cioAB on pMMB67EH | This study |
| pUC-cioAB | cioAB on pUC18 | This study |
| pUC-cydAB | cydAB of E. coli on pUC18 | This study |
a
Apr, ampicillin resistant; Cbr, carbenicillin resistant; Tcr, tetracycline resistant.