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Vector map of the ΔmurI mutant of Mycobacterium smegmatis mc2155. The construction of the delivery vector pKKYL02 was described in Materials and Methods. Homologous crossover at the left flank (LF) and right flank (RF) replaces 60% of murI with a nonpolar kanamycin resistance cassette (aphA-3). SmaI digestion sites utilized in the Southern blot are displayed. The right flank was used as a probe for hybridization.
