TABLE 3.
Growth of mutant and complemented strains on p-hydroxycinnamatesa
| Strain | Result |
||||||
|---|---|---|---|---|---|---|---|
| FA | CA | DFA | DCA | VA | DC | BA | |
| R. jostii Wt | + | + | + | + | + | + | + |
| R. jostii ΔcouL | − | − | − | − | + | + | + |
| R. jostii ΔcouL::pSETcouHTL | + | + | + | + | + | + | + |
| R. jostii ΔcouL::pSETcouHT | − | − | − | − | + | + | + |
| R. jostii ΔcouL::pSETcouTL | + | + | + | + | + | + | + |
| R. jostii ΔcouL::pSETcouL | − | − | − | − | + | + | + |
| R. jostii ΔcouL::pSET152 | − | − | − | − | + | + | + |
| R. jostii ΔcouO | − | − | − | − | + | + | + |
| R. jostii ΔcouO::pSETcouNO | + | + | + | + | + | + | + |
| R. jostii ΔcouO::pSETcouN | − | − | − | − | + | + | + |
| R. jostii ΔcouO::pSETcouNO_D257N | − | ND | ND | ND | + | ND | ND |
FA, ferulate; CA, p-coumarate; DFA, dihydroferulate; DCA, dihydro-p-coumarate; VA, vanillate; DC, dihydrocoumarin; BA, benzoate; Wt, wild type; +, growth rate and yield indistinguishable from wild-type growth; −, no growth detected; ND, not determined.