FIG 5.

Correlation between amyloidogenicity and kanamycin resistance in E. coli. (A) Polyglutamine tracts of three different lengths (20, 45, and 87 Q residues, designated by +Q20, +Q45, and +Q87) were directly fused at position 55 in APH, and the resulting sandwich fusions were expressed in NEB10β. Cell survival at maximum dilutions was scored for different concentrations of kanamycin. The Kan WT is strain AM144, Q20 is strain AM200, Q45 is strain AM201, and Q87 is strain AM308. (B) Western blots showing the distribution of polyQ sandwich fusion proteins in the soluble fraction (lanes S) and in the corresponding cell pellets of the strains (lanes P). Numbers on the left are molecular size markers (in kilodaltons).