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(A) OD600-versus-time plots for three replicate cultures of (i) the Δcel wild-type strain of M. extorquens PA1, (ii) the ΔmgsABC mutant, (iii) the ΔgmaS mutant, and (iv) the ΔmgsABC ΔgmaS double mutant in nitrogen-free medium with 3.5 mM succinate and 7.66 mM methylamine. (B) The topology of the N-methylglutamate pathway in M. extorquens PA1 is linear; NMGS is capable of synthesizing NMG from GMA as well as methylamine, albeit only in the ΔgmaS mutant.
