TABLE 1.
M. extorquens strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| CM2730 | M. extorquens PA1 Δcel | 39 |
| CM3733 | CM2730 ΔmgsABC | This study |
| CM3753 | CM2730 Δfae | 25 |
| CM3757 | CM2730 Δfae2 | This study |
| CM3761 | CM2730 Δfae3 | This study |
| CM3765 | CM2730 ΔgmaS | This study |
| CM3769 | CM2730 ΔmgdABCD | This study |
| CM3773 | CM2730 ΔftfL | 25 |
| CM3799 | CM2730 ΔglyA | 25 |
| CM3803 | CM2730 ΔmptG | 25 |
| CM3835 | CM2730 ΔmgsABC ΔgmaS | This study |
| CM4208 | CM2730 Δfae Δfae3 | This study |
| CM4230 | CM2730 Δfae Δfae2 | This study |
| CM4232 | CM2730 Δfae2 Δfae3 | This study |
| CM4244 | CM2730 Δfae Δfae2 Δfae3 | This study |
| Plasmids | ||
| pCM433 | Allelic exchange vector (Tetr Sucs) | 44 |
| pDN50 | pCM433 with Δfae upstream and downstream flanks | 25 |
| pDN51 | pCM433 with Δfae2 upstream and downstream flanks | This study |
| pDN52 | pCM433 with Δfae3 upstream and downstream flanks | This study |
| pDN53 | pCM433 with ΔmgsABC upstream and downstream flanks | This study |
| pDN54 | pCM433 with ΔgmaS upstream and downstream flanks | This study |
| pDN55 | pCM433 with ΔmgdABCD upstream and downstream flanks | This study |
| pRK2073 | Helper plasmid (IncP tra) | 53 |