FIG 3.

SadC is required for the hptB mutant hyperbiofilm phenotype. (A) Congo red binding of the indicated strains after 2 days of incubation. (B) Intracellular levels of c-di-GMP measured with the transcriptional PcdrA-gfp reporter. Relative fluorescence units (RFU) are arbitrary fluorescence intensity units corrected for cell density. At least three independent experiments were performed. Statistically significant changes are indicated (***, P < 0.0001, Student's t test). (C) PAK and PAK ΔsadC were conjugated with pBBR1-MCS4-ladS (pladS) or pBBR1-MCS4-hsbR (phsbR). Congo red binding is from day 2 of incubation.