TABLE 2.
Effect of the M171R substitution on FLAG-MurJ function
| Strain | Relevant genotype | Efficiency of plating ina: |
|
|---|---|---|---|
| LB | YT | ||
| NR2131 | ΔmurJ(pFLAGMurJΔCys) | 1.0 | 1.0 ± 0.3 |
| NR3075 | ΔmurJ(pFLAGMurJΔCys/M171R) | 0.0 ± 0.0 | <10−5 |
| NR3058 | ΔmurJ(pFLAGMurJΔCys/R24A/M171R) | 0.8 ± 0.5 | 0.4 ± 0.3 |
a
Efficiency of plating values for growth of haploid strains expressing FLAG-murJ alleles carrying the indicated substitutions were calculated as described in Materials and Methods. A value of 1 (set by the wild-type allele) indicates full complementation by FLAG-murJ alleles; a value of <1 indicates partial complementation. The haploid strain producing FLAG-MurJ/R24A is not viable, because the R24A substitution renders MurJ nonfunctional.