TABLE 2.
Functional classification of genes/open reading frames differentially expressed by overexpression of RpoE in H. ducreyi
| Regulation and function | No. of genesa |
|
|---|---|---|
| 5 min | 10 min | |
| Total differentially expressed genes | 180 (98% upregulated, 2% downregulated) | 312 (98% upregulated, 2% downregulated) |
| Upregulated genes | ||
| Envelope | ||
| Lipooligosaccharide biosynthesis and export | 13 | 21 |
| Outer membrane protein assembly and insertion | 4 | 4 |
| Peptidoglycan metabolism | 10 | 13 |
| Periplasmic chaperones and folding catalysts | 5 | 5 |
| Pilus | —b | 4 |
| Other envelope components | 9 | 10 |
| Cytoplasm | ||
| DNA mismatch repair | 8 | 17 |
| RNA modification | 8 | 10 |
| Regulation of gene expression | 12 | 13 |
| Cofactor biosynthesis | 7 | 14 |
| Amino acid biosynthesis | 1 | 3 |
| Monosaccharide metabolism | 1 | 5 |
| Stress adaptation | 3 | 3 |
| Miscellaneous | 1 | 2 |
| Hypothetical proteins | 95 | 182 |
| Downregulated genes | ||
| Envelope | 1 | 4 |
| Cytoplasm | 1 | 1 |
| Hypothetical proteins | 1 | 1 |
a
Number of genes differentially expressed in 35000HP(pDG9) compared to 35000HP(pDG10) at 5 or 10 min after induction of RpoE.
b
—, no genes were found to be differentially expressed.