FIG 3.
CRISPR3* adversely affects development in a RAMP-dependent manner. Five microliter samples of cell suspensions at 2.5 × 109 cells/ml of the indicated strains were spotted on CF plates. The photographs were taken after 5 days. Strains: WT (DK1622), CRISPR3* (BY850), ΔRAMP CRISPR3* (YZ1262), ΔpilA (DK10407), ΔpilA CRISPR3* (BY802), ΔpilA ΔRAMP CRISPR3* (YZ1261), ΔCRISPR3 (YZ1202), ΔpilA ΔCRISPR3 (YZ1200), and ΔpilA ΔCRISPR3 pXY105 (YZ1270). ΔRAMP (YZ1203) is wild type in development (not shown).