TABLE 1.
M. xanthus strains and plasmids
| Strain or plasmid | Genotype/description | Reference |
|---|---|---|
| Strains | ||
| BY802 | ΔpilA::tet CRISPR3* | This study |
| BY850 | CRISPR3* | This study |
| DK1622 | Wild type | 2 |
| DK10407 | ΔpilA::tet | 18 |
| DK10416 | ΔpilB | 44 |
| YZ601 | ΔdifA | 45 |
| YZ603 | ΔdifE | 46 |
| YZ811 | ΔsglK | 47 |
| YZ1200 | ΔpilA::tet ΔCRISPR3 | This study |
| YZ1201 | ΔpilA::tet ΔRAMP | This study |
| YZ1202 | ΔCRISPR3 | This study |
| YZ1203 | ΔRAMP | This study |
| YZ1261 | ΔpilA::tet ΔRAMP CRISPR3* | This study |
| YZ1262 | ΔRAMP CRISPR3* | This study |
| YZ1263 | ΔMXAN | This study |
| YZ1267 | ΔpilA::tet ΔMXAN | This study |
| YZ1270 | ΔpilA::tet ΔCRISPR3 att::pXY105 | This study |
| YZ1273 | ΔpilA::tet ΔMXAN CRISPR3* | This study |
| YZ1279 | ΔpilB CRISPR3* | This study |
| YZ1280 | ΔsglK CRISPR3* | This study |
| YZ1281 | ΔdifA CRISPR3* | This study |
| YZ1282 | ΔdifE CRISPR3* | This study |
| Plasmids | ||
| pMY7 | Cloning vector; Kanr; E. coli galK | Unpublished data |
| pMycoMar | magellan4 mutagenesis vector | 19 |
| pRW100 | CRISPR3 deletion in pMY7 | This study |
| pRW107 | MXAN deletion in pMY7 | This study |
| pRW112 | RAMP deletion in pMY7 | This study |
| pWB425 | M. xanthus expression vector | 20 |
| pXY105 | CRISPR3 in pWB425 | This study |
| pZErO-2 | Cloning vector; Kanr | Invitrogen |