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Degradation of antitoxins in trfA mutants. The SH1000 wild-type and ΔtrfA strains with either pALC6188 (mazEsa), pALC6486 (axe1), or pALC6489 (axe2) were grown to an OD650 of 1.1 with 0.1% xylose and then treated with rifampin (200 μg/ml). Cell lysates taken every 30 min were immunoblotted with an anti-GFP primary antibody, followed by a horseradish peroxidase-conjugated goat anti-mouse antibody. The experiments were repeated three times, and representative blots are displayed.
