Skip to main content
. 2014 Dec;196(23):4103–4110. doi: 10.1128/JB.02194-14

TABLE 1.

Genes for cloning and primers used for amplification

Gene Length (bp) Protein Molecular mass (Da) Primera
Orientation Sequence (5′→3′)
Celf_2726 2,283 CfXyl3A 82,018 Forward GGGGGGGAATTCCATATGCACCATCACCATCACCATGTGACGGATCTGAAGCTGTC
Reverse GGGGGGAAGCTTTCATCAGCGCACCACGGACGACGTCCAGCGCGCG
Celf_0140 2,277 CfBgl3A 82,305 Forward GGGGGGGAATTCCATATGCACCATCACCATCACCATGTGCCGCACCCGTACCAGGAC
Reverse GGGGGGAAGCTTTCATCAGGCGACGGTGAACCGGCCGCGGAGCAGGACCTC
Celf_0468 2,529 CfBgl3B 88,781 Forward GGGGGGGAATTCCATATGCACCATCACCATCACCATGTGTCGACGCAGACACCCCG
Reverse GGGGGGAAGCTTTCATCAGGGGGTGCCGGCCGCGGGGGCCGACGGG
Celf_3372 2,262 CfBgl3C 80,298 Forward GGGGGGGAATTCCATATGCACCATCACCATCACCATCCGCACGCGCCGTTCGACG
Reverse GGGGGGAAGCTTTCATCAGGCGCGGGCCTTCTCCACGAGCGCG
Celf_2783 1,454 CfBgl1 53,518 Forward GGGGGGGAATTCCATATGCACCAT CACCATCACCATACCACACGCGCCCC TCGG
Reverse GGGGGGAAGCTTTCATCAGGGCTG GTAGGTCGCGGCGTCCTCGGC
a

Restriction enzyme sites are underlined.