TABLE 2.
Summary of purification of β-glycoside hydrolases
| Enzyme | Purification step | Total activitya (U) | Total protein (mg) | Sp act (U/mg) | Purification (fold) | Recovery (%) |
|---|---|---|---|---|---|---|
| CfBgl1 | Crude enzyme extract | 189.5 | 141 | 1.34 | 1 | 100 |
| HiTrap Q HP column | 49.9 | 9.64 | 5.18 | 3.9 | 26.3 | |
| CfXyl3 | Crude enzyme extract | 94.6 | 124.8 | 0.76 | 1.0 | 100 |
| HisTrap HP column | 84.2 | 15.5 | 5.43 | 7.14 | 89 | |
| CfBgl3A | Crude enzyme extract | 286.1 | 179.1 | 1.60 | 1.0 | 100 |
| HiTrap HP Q column | 100.0 | 7.1 | 14.08 | 8.8 | 35.0 | |
| CfBgl3B | Crude enzyme extract | 8.0 | 73.5 | 0.11 | 1.0 | 100 |
| HiTrap HP Q column | 2.7 | 5.1 | 0.53 | 4.8 | 33.8 | |
| CfBgl3C | Crude enzyme extract | 103.2 | 97.0 | 1.06 | 1.0 | 100 |
| HiTrap HP Q column | 42.0 | 12.8 | 3.28 | 3.1 | 40.7 |
a
All the activities are reported as activity on pNP-β-Glc, except for CfXyl3A, which was assayed with pNP-β-Xyl.