FIG 3.

Dynamin-mediated endocytosis regulates cell surface MHC-I. (A) After entry of vAb4G into NBL6 cells, incubation was continued in the presence of increasing amounts of dynasore (2, 20, and 80 μM). Statistical significance was analyzed using one-way ANOVA. (B) NBL6 cells were transfected with WT dynamin (DynII; top) or its DN mutant (DynII-K44A; bottom), both of which are EGFP tagged. Twenty-four hours after transfection, cells were infected with the Ab4-dsRed2 virus. At 5 h p.i., infected cells in which dsRed2 was expressed were gated and analyzed using both EGFP (control or dominant negative) and Alexa Fluor 647 (MHC-I) filters. Differential cell populations are highlighted in the ellipses. Results are representative of three replicates.