FIG 3.

Structure of bicistronic minigenomes and transcribed mRNAs. (A) Scheme of Bicis containing the gene borders between the VP30 and VP24 genes (Bici VP30/VP24-wt IR 144nt) and the NP and VP35 genes (Bici NP/VP35-wt IR 5nt). The Bicis and sequences are shown in negative-sense orientation (not to scale). Regulatory sequences are illustrated as in Fig. 1A. The black boxes in Bici VP30/VP24-wt IR 144nt represent the ORFs of the reporter genes. The Bicis differ only in the gene border regions and the 3′- and 5′-UTR sequences flanking the gene borders. (B) Scheme of the three different mRNAs transcribed from a Bici template. (C) Calculated lengths of the mRNAs synthesized from the different Bicis, excluding the poly(A) tails.