FIG 4.

gH-null virus can infect 293 cells expressing gHgL but not cells expressing gB. 293 cells were transfected with empty vector (A and D) or with plasmids expressing gB (B) or gHgL (C), and 36 h later they were infected with Akata-gH-null virus (A, B, and C) or Akata-GFP virus (D). At 48 h postinfection, glycoprotein expression was detected using monoclonal antibody CL55 to gB (B) or monoclonal antibody CL59 to gHgL (C) and goat anti-mouse antibody conjugated to Alexa Fluor 647 (A to D), and infection was monitored in terms of GFP expression.