FIG 8.

Proteolytic digestion pattern of gB in virus incubated with soluble αvβ8 or treated with heat. (A, left) Aliquots of virus were held at room temperature, incubated at 45°C for 30 min, or incubated at room temperature with 6 nM soluble αvβ8. All but one of the aliquots held at room temperature were digested with 10 μg/ml proteinase K for 10 min, and all aliquots were analyzed by SDS-PAGE and Western blotting with antibody to gB. The blot is representative of those obtained in three independent experiments. (Right) Two aliquots of virus were incubated at 45°C for 20 min. One was digested immediately, and the other was incubated at 37°C for 120 min before digestion. The blot is representative of 5 independent experiments. (B) Four aliquots of virus were incubated for 30 min at the temperatures indicated, digested with proteinase K, and analyzed by SDS-PAGE and Western blotting with antibody to gB. The blot is representative of 4 independent experiments. (C) Four aliquots of virus were incubated at room temperature with soluble αvβ8 at the concentrations indicated, digested with proteinase K, and analyzed by SDS-PAGE and Western blotting with antibody to gB. The blot is representative of 2 independent experiments.