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. 2014 Nov;88(21):12222–12232. doi: 10.1128/JVI.02183-14

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Copyright © 2014 Nunes et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.

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FIG 3 — A synthetic virus with a chimeric BTV-1/BTV-8 VP2 is replication competent and cross-reacts with BTV-1 and BTV-8 antisera. (A) Protein alignment of BTV-1 and BTV-8 VP2. (B) The plaque morphology of parental and chimeric synthetic reassortants [_B1BTV-1^{(BTV-8-220-429)}] is shown (left). Neutralization assays (right) of BTV-1, BTV-8, and _B1BTV-1^{(8-VP2-220-429)} using BTV-1 and BTV-8 antisera were performed as described in Materials and Methods. BTV-1 and BTV-8 are neutralized only by homologous antisera. On the other hand, _B1BTV-1^{(8-VP2-220-429)} is neutralized by both BTV-1 and BTV-8 antisera.