TABLE 2.

Comparisons among different EV71 mouse models

Mouse straina	Source of virus	Age	Dose	Infectionb by:		Death	Limb paralysis	Skin rash	Spleen fibrosis	VP1 detection by IHC	Hematological abnormalities	Leukocyte infiltration
				Nonoral routec	Oral route
ICR	Serial passage adaptive	1 day–1 wk	2 × 105–5 × 106 PFU	+	+	+	+	−	−	Brain, spinal cord	ND	ND
ip-10 KO	Serial passage adaptive	2 wk	3 × 105 PFU	+	ND	+	+	−	−	Brain	ND	ND
AG129	Clinical isolate	2 wk	5 × 105–107 PFU	+	+	+	+	−	−	Brain stem	ND	ND
pEF-1α-hSCARB2 Tg	Clinical isolate	1 wk	3 × 104–107 PFU	+	ND	+	+	−d	−	Brain stem, spinal cord, intestine, muscle, skin	ND	ND
pSC2-hSCARB2 Tg	Clinical isolate	3–6 wk	102–106 TCID50	+	2/50e	+f	+f	−	−	Pons, medulla, cerebellar nuclei, spinal cord	ND	CD3+ T cells in brain, spinal cord, intestine, lung, muscle, skin
NOD/SCID	Clinical isolate	1 wk	107 PFU	+	+	+	+	+	+	Spleen, muscle, skin	+g	Macrophages in muscle
stat-1 KO	Clinical isolate	1 wk	108 PFU	+	ND	−	+	−	+	Pons, Purkinje layer, superior colliculus, spinal cord	ND	ND

^aSee reference 8 for ICR mice, reference 27 for ip-10 KO mice, reference 12 for AG129 mice, reference 17 for pEF-1α-hSCARB2-Tg mice, and reference 16 for pSC2-hSCARB2-Tg mice.

^bND, not done.

^cIncluding the intraperitoneal, intracerebral, intravenous, and subcutaneous routes.

^dHair loss associated with scurf, but no skin rash.

^eNumber of mice infected/total number of mice in a total of three experiments in which 1/20, 1/20, and 1/10 mice were infected, respectively.

^fAtaxia, paralysis, and death are defined as the same event in reference 16.

^gSee Fig. 4. The number of neutrophils and the red blood cell distribution width were increased, and the numbers of lymphocytes and eosinophils, mean cell volume, and mean cell hemoglobin were decreased, in EV71-infected mice.