TABLE 2.

Patterns of binding to recombinant proteins and functional activities of DENV-reactive neutralizing MAbs^a

^a*, lowest concentration at which 50% neutralization (Neut) occurred in flow cytometry-based neutralization assay against any of 4 serotypes. #, presence of >25-fold Ab-dependent enhancement for any of the 4 serotypes tested at 1 μg/ml. **, the key to phenotypes is as follows: D, dengue virus binding [the superscript indicates the serotype(s) of virus bound in whole-virus-capture ELISA]; Ag, antigen binding pattern, i.e., which recombinant E protein domain antigen, if any, was bound in ELISA (E = envelope, V = virion-only binding, DI-II = domain I-II fusion protein, and DIII = domain III); N, neutralization (the superscript indicates which of the 4 serotypes of virus were neutralized in the flow cytometric neutralization assay) (+, ++, and +++ indicate concentrations where 50% of virus was neutralized, in ranges of 1 to 5, 0.1 to 0.9, and <0.1 μg/ml, respectively); and E, enhancement, i.e., the serotype(s) of virus enhanced in a flow cytometric ADE assay at 1.0 μg/ml (−, <25-fold enhancement; +, 25- to 49-fold enhancement).