TABLE 3.

Pattern of binding to recombinant proteins and functional activities of DENV-reactive nonneutralizing MAbs^a

^a*, 50% neutralization was not detected at any concentration up to 5 μg/ml in flow cytometry-based neutralization assays against any of the 4 serotypes. #, presence of >25-fold Ab-dependent enhancement for any of the 4 serotypes tested at 1 μg/ml. **, the key to phenotypes is as follows: D, dengue virus binding [the superscript indicates the serotype(s) of virus bound in whole-virus-capture ELISA]; Ag, antigen binding pattern, i.e., which recombinant E protein domain antigen, if any, was bound in ELISA (E = envelope protein, prM = premembrane protein, DI-II = domain I-II fusion protein, and DIII = domain III); N, neutralization; and E, enhancement, i.e., the serotype(s) of virus enhanced in a flow cytometric ADE assay at 1.0 μg/ml (−, <25-fold enhancement; +, 25- to 49-fold enhancement; ++, 50- to 100-fold enhancement).