FIG 1.

SVV infection results in changes in immune cell populations in the lungs. (A) Viral loads from BAL and WB samples were measured by qPCR using primers and probes specific for SVV ORF 21 and are shown as average copy numbers per 100 ng of DNA (***, P < 0.001). Dashed line indicates limit of detection. The absolute numbers (means ± standard errors of the means [SEM]) of neutrophils and lymphocytes were quantified from BAL supernatant per 50 ml (B) and WB per 1 ml (C) ($$, P < 0.01; $$$, P < 0.001 for lymphocytes (+, P < 0.05; ++, P < 0.01 for neutrophils). The frequencies of CD4 T, CD8 T, and CD20 B cells (means ± SEM) were determined in BAL samples (D) and PBMC (E) by flow cytometry (#, P < 0.05; ####, P < 0.0001 for CD8 T cells) (&&&&, P < 0.0001 for CD20 B cells).