TABLE 1.
Characteristics of IgG from each human serum sample
| Sample | HSV status | Sheddinga | Neutralization (μg/ml IgG)b |
Reaction with glycoproteinc |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HSV-1 | HSV-2 | gB-1 | gB-2 | gC-1 | gC-2 | gD-1 | gD-2 | gHgL-1 | gHgL-2 | |||
| 1 | 1, 2 | Low | 23 | 90 | +++ | +++ | +/− | + | + | +++ | + | − |
| 2 | 2 | Low | 125 | 90 | +++ | +++ | − | +++ | + | +++ | +/− | − |
| 3 | 1, 2 | Low | 125 | 190 | +++ | +++ | − | + | + | +++ | + | − |
| 4 | 2 | Low | 23 | 18 | +++ | +++ | +/− | +++ | +++ | +++ | + | − |
| 5 | 2 | Low | 30 | 12 | ++ | +++ | − | +++ | + | +++ | − | + |
| 6 | 1, 2 | High | 8 | 5 | +++ | +++ | +++ | +++ | +++ | +++ | ++ | − |
| 7 | 1, 2 | High | 7 | 12 | +++ | +++ | − | +++ | + | +++ | − | − |
| 8 | 2 | High | 55 | 38 | +++ | +++ | − | +++ | ++ | +++ | − | − |
| 9 | 1, 2 | High | 3 | 10 | +++ | +++ | +/− | + | ++ | +++ | + | + |
| 10 | 1, 2 | High | 9 | 7 | +++ | +++ | +++ | +++ | +++ | +++ | ++ | + |
a
As determined by Tronstein et al. (23). “High” shedding rates are defined as detection of HSV on >10% of days, while “low” shedding rates are defined as detection of HSV on <10% of days.
b
Values represent a 50% reduction in plaques as compared to the control (no IgG).
c
Determined by IgG reactivity to purified proteins via Western blotting (Fig. 2). +++, very strong; ++, strong; +, moderate; +/−, weakly positive; −, negative.