FIG 6.

Binding of the full-length SA11 NSP2 to the peptide array of the SA11 VP2 sequence. (A) Autoradiograph of the VP2 peptide array probed by full-length NSP2. The peptide array consists of spots of 17-residue peptides in the protein sequence, starting from the N terminus (spot A1) and ending with the C-terminal peptide (J21), with the N-terminal residue of the peptide in each spot shifted by 2 residues from the previous spot along the protein sequence. (B) Graph showing relative intensity (y axis) of each spot (black bars) in the array with its position relative to the protein sequence (x axis). (C) The peptide sequences corresponding to the spots denoted 1 to 21 in panel B that showed intensities higher than the 60% cutoff. VP2 residues present in at least two peptides that showed ≥80% binding intensity (green) were mapped onto the known structure of VP2 (pdb 3KZ4). (D) Surface model of the VP2 dimer, with NSP2-binding sites (green) labeled on monomer A (yellow) and monomer B (olive). The N-terminal domain is indicated by a black arrow.