FIG 7.
Binding of the SA11 VP21-134 to the peptide array of the SA11 NSP2 sequence. (A) Autoradiograph of the NSP2 peptide array probed by full-length VP21-134. The peptide array consists of spots of 17-residue peptides in the protein sequence, starting from the N terminus (spot A7) and ending with the C-terminal peptide (F16), with the N-terminal residue of the peptide in each spot shifted by 3 residues from the previous spot along the protein sequence. (B) Graph showing relative intensity (y axis) of each spot (black bars) in the array with its position relative to the protein sequence (x axis). (C) The peptide sequences corresponding to the spots denoted 1 to 10 in panel B that showed intensities higher than the 60% cutoff. NSP2 residues present in at least two peptides that showed ≥80% binding intensity (green) were mapped onto the known structure of NSP2 (pdb 1L9V). (D) Surface model of the NSP2 octamer (gray) with VP21-134-binding residues shown in green on monomer A (pink).