FIG 6.

SCD1 recruits both NS5A and NS4B proteins and enriches them on the detergent-resistant membrane fraction. (A) Huh7.5 cells were transfected with either negative siRNA or siRNA targeting SCD1 for 24 h. Cells were then further transfected with Myc-tagged NS5A for 48 h. Total cell lysates were separated into either detergent-soluble or detergent-resistant membrane fractions as described in Materials and Methods. Cell lysate, detergent-soluble (DS), and detergent-resistant (DR) membrane fractions were analyzed by immunoblot analysis with the indicated antibodies. (B) Huh7.5 cells were treated with SCD1 inhibitor for 24 h. Cells were then transfected with Myc-tagged NS4B and incubated for 48 h in the presence of SCD1 inhibitor. Cell lysate, detergent-soluble (DS), and detergent-resistant (DR) membrane fractions were analyzed as described for panel A.