FIG 6.

ANTS/DPX fusion assay with HAV VP4 and FHV γ1. A mixture of liposomes containing 100 mM the fluorescent dye ANTS and 200 mM the quencher DPX was either left untreated or treated with 50 μM VP4, FHV γ1, or 0.5% Triton X-100, and the fluorescence of ANTS was monitored continuously for 20 min at an emission wavelength of 520 nm. No decrease in ANTS fluorescence was detected at any point, suggesting leakage, but not fusion, of vesicles.