FIG 1.

MHV68 latency alters initial LCMV replication. Ten-week-old naive C57BL/6 mice were intranasally (i.n.) infected with 10⁴ PFU of MHV68 or medium. Thirty-five days postinfection, mice were challenged with serum-free RPMI or 2 × 10⁵ PFU of LCMV Armstrong intraperitoneally (i.p.). On days 1, 2, 3, 8, and 10 post-LCMV infection the spleen (A), liver (B), lung (C), and kidney (D) were harvested. Viral titers were determined by plaque assay, and the average and standard deviation are plotted. *, significant difference between mice infected with LCMV and mice infected with both LCMV and MHV68 (P ≤ 0.05). Six to nine mice were harvested in two to three independent experiments.