FIG 1.

Purification of NSP4 WT-CCD and the CCD mutants. (A) Sequence alignment of strain SA11 NSP4 residues 95 to 146 of WT-CCD and the E120A-CCD and E/Q-CCD mutants showing specific amino acid mutations in WT-CCD, indicated with asterisks. The strain ST3 NSP4 CCD is also shown in the alignment to show that the E120 and Q123 residues are conserved. (B) SDS-PAGE gel of the NSP4 WT-CCD, E120A-CCD, and E/Q-CCD purified proteins with His tag (+His) and without His tag (−His). Molecular mass marker bands (lane M) are shown on left of the gel.