TABLE 1.
Sequences of the mutagenic primersa
| Primer name | DNA sequence (5′-3′) |
|---|---|
| EBV gH_C120A-F | CTGAATTCCCCTGCCGCCATGCTTAGTGCCCC |
| EBV gH_C278A-F | GATGAAGGGAGGCGCCCGGGAGCCGGAA |
| EBV gH_C120S-F | CTGAATTCCCCTGCCAGCATGCTTAGTGCCC |
| EBV gH_C278S-F | TGAAGGGAGGCAGCCGGGAGCCG |
| EBV gH_C312A-F | GACTGGCAATGGCGCCGTGGACCTCCGC |
| EBV gH_C335A-F | GAAAGACATCATCGGCATAGCTTATGGGGCCACTGTCAAG |
| EBV gH_C312S-F | GACTGGCAATGGCAGCGTGGACCTCCG |
| EBV gH_C335S-F | AAGACATCATCGGCATAAGTTATGGGGCCACTGTC |
| EBV gH_E273A-F | CTGGTCCTGCTGGCGATGAAGGGAGGC |
| EBV gH_R279A-F | GAAGGGAGGCTGCGCGGAGCCGGAACTG |
| EBV gH_E280A/P281A/E282A-F | GAGGCTGCCGGGCGGCGGCACTGGACACGGA |
| EBV gH_G333A/I334A-F | GTCCTGAAAGACATCATCGCCGCATGTTATGGGGCCACTGTC |
| EBV gH_Y336A-F | CCTGAAAGACATCATCGGCATATGTGCTGGGGCCACTGT |
| EBV gH_Y336F-F | GAAAGACATCATCGGCATATGTTTTGGGGCCACTGT |
a
The sequences of the mutagenesis primers are shown, and the mutated nucleotides are underlined. Since the forward (F) and reverse primers are complementary, only forward primers are shown.