FIG 1.

Antibodies against thrombin and other coagulation factors in DENV-immunized mouse sera. (A) Mice were immunized with DENV antigens as described in Materials and Methods. Serum samples were collected on day 32. Antibodies bound to DENV antigens, rNS1, and human thrombin were detected by ELISA. (B) Different concentrations of DENV antigens, rNS1, or BSA were preincubated with DENV-immunized mouse sera before their addition to human thrombin-coated ELISA plates. Bound antibodies were detected as described in Materials and Methods. (C) Antibodies in the DENV-immunized mouse sera were bound to various human coagulation factors, as determined using different coagulation factor-coated ELISA plates. The results are presented as the means ± standard deviations from three independent experiments.