FIG 2.

Characterization of rabbit ATAs. (A) Western blotting was performed to assess the ability of the rabbit ATAs to bind to DENV protein, Plg, human/bovine thrombin (top panel), and recombinant NS1 proteins of different lengths as indicated (bottom panel). Lane 1, C6/36 cell lysate; lane 2, DENV-infected C6/36 cell lysate; lane 3, supernatants from DENV-infected C6/36 cells; lane 4, PEG-precipitated DENV antigens; lane 5, Plg; lane 6, bovine thrombin; lane 7, human thrombin. (B) Ability of rabbit ATAs to bind to bovine/human thrombin, Plg, and BSA as determined by ELISA. (C and D) Rabbit ATAs were preadsorbed to BSA-, NS1-, Plg-, or thrombin-conjugated Sepharose as indicated. The ability of the nonadsorbed antibodies to bind to thrombin or Plg was determined by ELISA. The results are presented as the means ± standard deviations from three independent experiments.