FIG 4.

ATAs and MAbs from DENV-immunized animals inhibit thrombin activity in vitro. (A) Human thrombin was preincubated with ATAs, MAbs (7D2 and 6H11), control rabbit Igs (RaIg), control mouse IgG (mIgG), or control mouse IgM (mIgM) for 1 h, and thrombin activity was determined by S-2238. (B) Human thrombin was preincubated with antibodies for 1 h before its addition to human PPP. Fibrin formation was detected by the change in turbidity at OD₃₅₀. The data are presented as the means ± standard deviations from three independent experiments. Significance was analyzed by two-way ANOVA (*, P < 0.05).