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. 2014 Dec;88(23):13689–13698. doi: 10.1128/JVI.02035-14

FIG 6.

FIG 6

DDX3 knockdown leads to increased HBV RNA levels. (A) The effect of DDX3 knockdown in HBV-Tof cells. HBV-Tof cells stably expressing shDDX3 or shGFP were cultured in doxycycline-free medium and harvested for total RNA extraction at the indicated time points. HBV transcripts were analyzed by Northern blotting, with ribosomal RNAs serving as the loading control. The intensity of viral RNAs from HBV-Tof cells (lanes 1, 2, 3, and 4) was set to 100%. Endogenous DDX3 expression was evaluated by Western blotting using anti-DDX3 antibody. (B) The effect of DDX3 knockdown in HepG2.2.15 cells. Total RNAs were extracted from HepG2.2.15 cells stably expressing shDDX3 or shGFP and analyzed by real-time RT-PCR. Western blot analysis was performed to confirm efficient DDX3 knockdown in HepG2.2.15 cells.