FIG 4.

One-step growth curve of HSV-1 in cholesterol-deficient cells. DHCR24^−/− cells (A, B), DHCR24^+/+ cells (A), or DHCR24^−/− cells supplemented with SyntheChol (B) were infected with HSV-1 KOS at MOIs of 5 to 10 for 1 h at 37°C. Cells were then rinsed with warm PBS and replenished with fresh medium. Triplicate samples were collected at different time points over a 24-h period, and plaque titers determined on Vero cells. Data are mean results of triplicate determinations with standard deviations.