Evaluation of the EEHV5A-5B chimeric domain patterns and boundaries relative to EEHV2. The diagrams show SimPlot comparisons between EEHV5A, EEHV5B, and EEHV2 across all three EEHV5B chimeric domain regions, including all of CD-I (2.4 kb) and CD-II (1.1 kb) and the left-hand side boundary of CD-III (>2.7 kb). (a) CD-I. The 4,800-bp U39(gB)-U38(POL) segment from across map coordinates 74,002 to 78,861 for EEHV5A(NAP50) compared to EEHV5B(NAP58) (blue) and to EEHV2(NAP12) (red) is shown. (b) CD-II. The 2.8-kb U48(gH)–U48.5(TK)–U49–U50 segment from across map coordinates 105,396 to 108,178 for EEHV5A(NAP50) compared to EEHV5B(NAP58) (blue) and to EEHV2(NAP12) (red) is shown. (c) CD-III. The left-hand side only of the 4,300-bp segment across map coordinates 143,644 to 147,958 for EEHV5A(NAP50) encompassing U77(HEL C terminus), U77.5(ORF-M), U80.5(ORF-N), U81(UDG), U82(gL), and U82.5(ORF-Oex3) compared to EEHV5B(NAP58) (blue) and EEHV2(NAP12) (red) is shown.