TABLE 4.
DNA and protein divergence in ORFs across the EEHV1A, EEHV1B, and EEHV6 genomesa
| Gene locus | EEHV1A(Kimba) coordinatesb | Protein sizeb | Nucleotide level divergence (%)c |
Amino acid level divergence (%)c |
Chimeric domain | ||||
|---|---|---|---|---|---|---|---|---|---|
| 1A-1B | 1A-6 | 1B-6 | 1A-1B | 1A-6 | 1B-6 | ||||
| U39(gB) | 73959–76511 | 836 | 21 | 16 | 20 | 14 | 11 | 13 | CD-I |
| U38/(POL) | (76544–79043) | (832) | 5 | 17 | 16 | 4 | 12 | 12 | CD-I |
| U33 | (83628–84428) | (266) | 1.3 | 22 | 21 | 0.3 | 19 | 18 | |
| U28(RRA) | 99358–99760 | 801 | 1.1 | 14 | 15 | 0 | 8 | 8 | |
| U27.5(RRB) | 99804–100709 | 302 | 1.4 | 14 | 14 | 0.3 | 17 | 17 | |
| U27(PPF) | 100960–102186 | 408 | 1.8 | 16 | 16 | 0.5 | 6 | 6 | |
| U45.7(ORF-J) | 102168–102775 | 168 | 26 | 16 | 25 | 32 | 14 | 33 | CD-II |
| U46(gN) | 102759–103713 | 96 | 29 | 16 | 28 | 20 | 6.2 | 18 | CD-II |
| U47(gO) | 103075–103713 | 212 | 35 | 16 | 35 | 38 | 11 | 37 | CD-II |
| U48(gH) | (105363–105903) | (179) | 31 | 17 | 28 | 33 | 21 | 37 | CD-II |
| U48.5(TK) | 105835–106908 | 356 | 13 | 16 | 18 | 16 | 21 | 20 | CD-II |
| U49 | 106910–107608 | 232 | 3.6 | 15 | 15 | 0.4 | 12 | 13 | |
| U50(PAC2) | (107427–108083) | (220) | 4.0 | 23 | 23 | 3.1 | 20 | 21 | |
| U51(vGPCR1) | (109398–110239) | (286) | 4.2 | 22 | 23 | 3.3 | 14 | 17 | |
| U57(MCP) | (115577–117866) | (748) | 2.7 | 17 | 17 | 1.2 | 7 | 7 | |
| U60(TERex3) | 123601–124175 | (194) | 2.9 | 12 | 13 | 0d | 0d | 0d | |
| U62 | 124231–124477 | 88 | 0.8 | 13 | 13 | 0 | 9 | 9 | |
| U71(MyrTeg) | 132954–133241 | 96 | 8 | 25 | 25 | 9 | 23 | 26 | |
| U72(gM) | (133320–133614) | (97) | 4.4 | 13 | 14 | 3.3 | 9.2 | 7.2 | |
| U73(OBP) | (134615–135415) | (277) | 3.0 | 14 | 14 | 1.9 | 6.0 | 6.7 | |
| U76(POR) | (139998–141295) | (433) | 1.9 | 13 | 13 | 0 | 4.2 | 4.2 | |
| U77(HEL) | (141294–141864) | (190) | 2.8 | 12 | 13 | 0.5 | 7.0 | 7.5 | |
| U77.5(ORF-M) | 143988–145502 | 503 | 16 | 16 | 19 | 13 | 13 | 16 | CD-III |
| U80.5(ORF-N) | 145642–145959 | 106 | Del | 31 | Del | Del | 29 | Del | CD-III |
| U81(UDG) | 146027–146980 | 317 | 29 | 19 | 30 | 29 | 19 | 30 | CD-III |
| U82(gL) | 146946–147860 | 304 | 31 | 18 | 30 | 33 | 22 | 41 | CD-III |
| U82.5(ORF-O) | (147700–147910) | (78) | 33 | 16 | 31 | 34 | 18 | 37 | CD-III |
| U85.5(ORF-K) | (152200–153350) | (380) | 1.7 | 26 | 26 | 0.8 | 25 | 25 | |
| U86.5(ORF-L) | (155324–158615) | (1084) | 2.4 | 11 | 11 | 0.6 | 18 | 18 | |
a
The rows in the table shown in bold type indicate ORFs that are highly variable between EEHV1A and EEHV1B.
b
The EEHV1A coordinates are based on Ling et al. (13). The values shown in parentheses indicate that an incomplete ORF or shorter region was used.
c
The divergence between EEHV1A and EEHV1B (1A-1B), EEHV1A and EEHV6 (1A-6), and EEHV1B and EEHV6 (1B-6) is shown. Del, ORF-N is absent (deleted) in all EEHV1B strains.
d
All three TER proteins are identical or only 1 aa different over this 194-aa segment.