TABLE 5.
DNA and protein divergence in ORFs across EEHV5A, EEHV5B, EEHV2, and EEHV1Aa
| Gene locus | EEHV1A coordinatesb | Protein sizeb | Nucleotide level divergence (%)c |
Amino acid level divergence (%)c |
Chimeric domain | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 5A-5B | 5A-2 | 5B-2 | 5A-1 | 5A-5B | 5A-2 | 5B-2 | 5A-1 | ||||
| U39(gB) | (74091–76511) | (837) | 10 | 16 | 15 | 27 | 4.2 | 7.6 | 6.3 | 21 | CD-I |
| U38(POL) | (76583–79038) | (818) | 1.3 | 18 | 17 | 24 | 1.4 | 11 | 11 | 21 | CD-1 |
| U33 | (83648–84183) | (176) | 0.8 | 28 | 28 | 37 | 0 | 25 | 25 | 43 | |
| U28(RRA) | (98637–99760) | (374) | 0.4 | 17 | 17 | 23 | 0 | 8.6 | 8.6 | 16 | |
| U27.5(RRB) | 99804–100709 | (301) | 0.9 | 16 | 16 | 24 | 0.7 | 6.3 | 6.3 | 15 | |
| U27(PPF) | (100960–101609) | (216) | 0.8 | 19 | 19 | 31 | 0.9 | 14 | 14 | 25 | |
| U48(gH) | (105396–105911) | (172) | 0 | 18 | 18 | 38 | 0 | 16 | 16 | 34 | |
| U48.5(TK) | 105835–106908 | 344 | 14 | 18 | 20 | 30 | 13 | 19 | 19 | 27 | CD-II |
| U49 | 106910–107628 | 233 | 6.0 | 11 | 10 | 24 | 2.1 | 6.9 | 4.7 | 24 | CD-II |
| U50(PAC2) | (107428–108178) | (261) | 0.2 | 18 | 18 | 32 | 0 | 19 | 19 | 34 | |
| U51(vGPCR1) | (109225–110065) | (292) | 4.6 | 22 | 23 | 32 | 2.7 | 17 | 17 | 25 | |
| U57(MCP) | (116671–117809) | (380) | 0.2 | 19 | 19 | 27 | 0.3 | 9.2 | 9.2 | 19 | |
| U60(TERex3) | (123674–124180) | (169) | 1.2 | 13 | 13 | 21 | 0 | 1.3 | 1.3 | 3.0 | |
| U62 | (124231–124434) | (75) | 0.5 | 16 | 16 | 29 | 0 | 10 | 10 | 19 | |
| U71(MyrTeg) | 132954–133241 | 98 | 8.7 | 24 | 23 | 44 | 14 | 27 | 30 | 56 | |
| U72(gM) | 133316–133613 | (101) | 0.3 | 16 | 17 | 21 | 0 | 10 | 10 | 12 | |
| U73(OBP) | (134605–134430) | (269) | 0 | 12 | 12 | 21 | 0 | 4.4 | 4.4 | 10 | |
| U76(POR) | (139815–141295) | (493) | 0.2 | 13 | 13 | 22 | 0.4 | 4.1 | 4.1 | 7.8 | |
| U77(HEL) | (141294–141793) | (181) | 0.4 | 14 | 14 | 24 | 0.7 | 6.0 | 6.0 | 14 | |
| U77.5(ORF-M) | 143988–145511 | 484 | 1.1 | 16 | 17 | 29 | 0 | 10 | 10 | 17 | CD-III |
| U80.5(ORF-N) | 145642–145959 | 106 | 28 | 23 | 27 | 38 | 32 | 24 | 33 | 47 | CD-III |
| U81(UDG) | 146027–146980 | 324 | 27 | 23 | 28 | 33 | 23 | 21 | 20 | 29 | CD-III |
| U82(gL) | 146946–147776 | 312 | 27 | 17 | 27 | 34 | 26 | 16 | 26 | 44 | CD-III |
| U82.5(ORF-Oex3) | 147694–147958 | (88) | 23 | 21 | 25 | 32 | 21 | 20 | 20 | 25 | CD-III |
| U82.5(ORF-O) | 147700–148985 | 487 | 36 | 54d | 34 | 53d | |||||
| U83.5(ORF-P) | 146932–150638 | 526 | 33 | 48d | 33 | 52d | |||||
| U85.5(ORF-K) | 152042–154436 | (678) | 1.3 | 23 | 22 | 41 | 0.4 | 29 | 27 | 48 | |
| U86.5(ORF-L) | 155053–155356 | (97) | 1.4 | 10 | 12 | 30 | 1.0 | 7.0 | 8.4 | 20 | |
a
The prototype genomes used were EEHV5A(NAP50), EEHV5B(NAP58), EEHV2(NAP12), and EEHV1A(NAP18). The rows in the table shown in bold type indicate ORFs with high-level variability (hypervariable genes) between EEHV5A and EEHV5B.
b
The values in parentheses indicate that incomplete ORFs were used.
c
The divergence between EEHV5A and EEHV5B (5A-5B), EEHV5A and EEHV2 (5A-2), EEHV5B and EEHV2 (5B-2), and EEHV5A and EEHV1 (5A-1) is shown.
d
These values show divergence between EEHV5B and EEHV1A.